Knowledge Base: pH Test Strips

Knowledge Base: pH Test Strips2020-02-19T15:08:34-07:00
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At what pH value does the blue litmus paper change from blue to red?2020-02-14T12:27:39-07:00

We have tested the blue litmus with multiple pH buffers. The blue paper is blue at pH 8. At pH 7, the sample is maybe just a little red. The real change to red seems to start with pH 6.5 and lower.

Can I test the pH of my body with litmus papers?2020-02-14T12:29:46-07:00

No. Litmus papers are qualitative strips, meaning they employ a color change, not a specific color chart, to indicate whether a water sample is acidic, basic or neutral. The specific pH value is not determined.

Can I use pH test strips in non-buffered or low-buffered aqueous solutions?2020-02-14T12:29:36-07:00

Currently, our pH test strips are designed for testing buffered solutions, and the calibrations (color charts) are made on buffers against a pH-meter measurement. For non- or low-buffered solutions, these strips are not suitable. For this purpose, there are special pH test pads designed for low-ionic and low-buffered waters, such as water in aquariums or pools. Please see our Aquarium and Pool test strips.

Does temperature affect the pH test strips?2020-02-14T12:26:48-07:00

Our pH Color charts were developed using NIST-traceable pH buffers at 25°C. Colorimetric pH test strips do not offer “temperature compensation.” Care should be used when comparing results using test strips to those obtained with a temperature compensated pH meter. It is well established that the “true pH” of a solution will be influenced by temperature. The extent of the effect is specific to the solution being tested. It is good practice to record the temperature of the solution when expressing results of pH testing. In the case of test strips, since they do not compensate for temperature, it is recommended that the sample being tested be brought to room temperature first.

How do you read 3-pad pH test strip results?2020-02-14T12:28:45-07:00

Typically, only two of the three pads changes color at any given pH level, which you match with the closest three blocks on the color chart. Changes can be subtle, and the colors may appear different under different lighting conditions (fluorescent, incandescent or sunlight). Three-pad pH test strips can provide for the most accurate results.

I’m testing the pH of RO, distilled, and tap water, but the test strips aren’t changing color. Are the strips not working or is my water off the charts?2020-02-14T12:29:08-07:00

When testing RO, distilled, or tap water, the strips may not behave like you would expect. The colors on the chart were developed for these strips using buffer solutions. The indicators used with the strips require that the waters being tested have some buffering capacity. RO and distilled waters have little or no buffering capacity, and the test strips will not work in these waters. If the tap water has a very low buffer capacity (low hardness, low alkalinity, etc.) the color can take much longer than the recommended 1-2 second immersion and 10-15 development time.

pH Test Strips & Litmus Paper Instructional Video2020-02-14T12:28:16-07:00

What pH test strips can I use to test kombucha tea?2020-02-14T12:28:05-07:00

Our Wine pH test strips, measuring from 2.8-4.4 work great for testing kombucha tea as well.

What test strips can be used to monitor pH during fermentation of wine?2020-02-14T12:28:56-07:00

Prior to, and during the process of fermentation, a measure of the pH of the solution is quite important. The correct pH must be established initially, and should be monitored throughout the process in order to facilitate the complete breakdown of the initial carbohydrates to alcohol. This measure of pH may be accomplished with the Universal pH test paper. In addition, the pH 1-14 test strips can be used to test the acidity (pH) of the juice/must/beer prior to and during the process of fermentation. For an initial measure of the pH of juice, a narrow range measure for wine production can be obtained using the pH 2.8-4.4 test strips.

What test strips should I use to measure mash and wort pH?2020-02-14T12:28:35-07:00

Monitoring the pH of the Mash, Wort, and Beer is necessary as the Yeast will develop differing physiological states, and the presence or absence of Organic Acids and the use of mineral Ions during the fermentation process will cause changes in acidity or alkalinity. This in turn affects Enzyme activity in the Yeast.

Prior to, and during the process of fermentation, a measure of the pH of the solution is quite important. The correct pH must be established initially, and should be monitored throughout the process in order to facilitate the complete breakdown of the initial carbohydrates to alcohol. This measure of pH may be accomplished with the Universal pH test paper. In addition, the pH 1-14 test strips can be used to test the acidity (pH) of the juice/must/beer prior to and during the process of fermentation. For an initial measure of the pH of the mash for beer, a narrow range measure can be obtained using the pH 4.6-6.2 test strips.

The pH of Yeast is best at 5.2-5.5. Malted Barley will release Phosphate Ions, and these will cause the water to be buffered at a pH of 5.6. Attaining an optimum pH range will ensure more complete conversion of starches in grain to fermentable sugars. In addition proper hop utilization will occur.

When I test the pH of red wine, it stains the test strip. How can I avoid this?2020-02-14T12:27:55-07:00

It is not a good idea to dilute the sample with water. While this helps to avoid the color stain, dilution of the hydrogen ion concentration will change the pH.

A better suggestion for measuring pH might be to use activated carbon (like that used in aquarium filters) to help take the color out of solution sample. This method doesn’t appear to affect the pH of the solution.

Why don’t my beer pH 4.6-6.2 Test Strips work?2020-02-14T12:27:04-07:00

1) The pads on the strips are yellow when they are made.  If they are not yellow then they have been exposed to heat, humidity, light, or some other detrimental condition.

2) The test strips will change color outside the pH range shown on the color chart. The strips will turn purple in alkaline waters.  There is a way to check this.   Concentrated bleach will turn the strips purple and then yellow and then white as the paper bleaches out.  Dilute bleach will turn the strips purple and then eventually a greenish color if they sit out for a few minutes.  In acids, there isn’t as much change.  When testing vinegar (dilute acetic acid) they should remain yellow.  I have tried the strips using both concentrated and dilute acetic acid – both were yellow.  You might try adding a little vinegar or bleach to small samples of the water to confirm that the strips react as expected.

3)  Temperature has a slight effect on the test results.  It seems that raising the temperature from 25C to 70C causes the color to shift about ½ a color unit higher.

4)  When testing RO, distilled, or tap water, the strips might not behave as one might expect.  The indicators used with the strips require that the waters being tested have some buffering capacity.  RO and distilled waters have little or no buffering capacity and the strips will not work in these waters.  If the tap water has a very low buffer capacity (low hardness, low alkalinity, etc.) the color can take much longer than the recommended 1-2 second immersion and 10-15 development time.  We ran a test to find results on various buffer solutions with the color transitioning from yellow to purple from pH 4-7 in 0.5 unit increments.  These particular buffers did not develop very intense colors in the pH 5-6 region.  The shades were close but the intensity was not as intense as the color chart.  We would expect that the mash has sufficient buffer capacity.

Since the color of the strip remained yellow, that would seem to indicate that the pH was lower than 4.5.  If this is not what you would expect we would suggest two things:

1)  Try the bleach test described above in item (2) to make sure the strips are reacting like they should.  If they do not turn purple, then it is possible that the strips are old.

2)  Try a fresh vial of strips.

Why don’t pH test strips require special packaging?2020-02-14T15:17:04-07:00

The pH test strips and papers are based on acidobasic indicators, which change their colors in a particular pH range. Acidobasic indicators are generally very stable compounds, and we have chosen the best indicators based on their properties for use in our test strips. They are so stable that special packaging (such as air-tight stoppers, unpermeable material for packing, dessicant, etc) is not necessary.

When stored, keep the strips out of direct sunlight. Sunlight can fade the color with time, like any other colored materials exposed to light for too long. The biggest issue in this situation is fading (bleaching) of the color scale on the label, which serves as a calibration for pH reading. Fading of the color scale on the label can occur faster than the fading of strips themselves.

Similarly, exposure to high humidity for a long period of time can also cause changes in the color reaction system. When the moisture dries on the paper, it causes fading or color changes.

Generally, the minimum expiration date for pH test strips and papers is 3 years when stored at 10 – 30C in a dry place, away from chemical fumes in laboratories.

Why is the Universal pH paper staining the color chart and vial?2020-02-14T12:27:24-07:00

The Universal pH test paper does undergo some sublimation over time. This is evidenced by the slight discoloration of the color chart and vial. We have found that this sublimation does not normally adversely affect the performance of the test paper.

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