When making a glucose solution, how long does it take for mutarotation to occur?

If you are preparing glucose standards in a lab to use with our Glucose Test Strips, in order for it to work properly, you must wait about 3 hours to allow mutarotation to occur.

Solid glucose is dissolved in water and over time (3 hours) undergoes mutarotation to the cyclic forms a-d-Glucose and b-d-glucose (equilibrates in a ratio of 36:64). These cyclic forms are what the glucose enzyme interacts with on the test paper.